2001/02/22: Updated to work with ImageJ 1.21
2001/10/23: Bug fixes
2001/02/22: Updated to work with ImageJ 1.26
2003/02/21: Bug fixes
2003/11/20: Fixed “Z Projection” option in FVControl
2010/08/12: Updated to work with ImageJ 1.43
A sample image from a
12 bit confocal microscope is available. It is a single plate with
three channels. Each channel is a separate 16 bit image, with a separate 8 bit LUT for display on the
Open the “FV Control” plugin.
Select “Open Fluoview Image” on the FV controller.
Select the sample image (fluoview.tif ).
Play with the FV control to turn various Active channels on and off.
Play with the ROV adjuster which is called from the FV control.
Use the “ExtendedInfo” plugin to read the original information on
collection parameters, as well as to show the various details of the 8
bit LUT used for the display of each channel.
Use the Split FV on the FV Control. Notice that each individual slice
retains the original 8 bit LUT
Call the “SynchWindows” plugin. Select the original image and all the
derived slices. Click on Synchronize all.
When you move a cursor on one image, it will move to the
corresponding location on all of the of selected images.
You can directly convert the original file to RGB 24 bit. However,
in the original format you could change the gray scale image with the
ROV control without altering the image of of any other channel. After
converting to RGB you will find that there is no way that you can
independently modify the gray scales, as it is now represented in the
R,G and B channels.
Author: Wayne Rasband (email@example.com) History: 2000/11/28: First version 2003/01/28: Uses the image or selection min and max; works correctly with signed images Source: SixteenBit_Histogram.java Installation:
Author: Paul Baggethun (Paul.Baggethun at ALCOA.COM) Date: 2002/06/10 Source: Microscope_Scale.java Installation: Download Microscope_Scale.class to the plugins folder and restart ImageJ. Description: This plugin lets you