Have the plugin Sync_Windows_1.3
or higher installed. Put Spectral_Unmixing.jar
into the ImageJ plugins-folder or a subfolder and restart ImageJ.
A submenu “SpectralUnmixing” of the Plugins menu appears, which contains the
entries “Display Mixing Matrix”, “Measure Mixing Matrix” and “Unmix”.
Spectral unmixing is an algorithm, which is applied to fluorescence images to
correct for spectral bleedthrough between different color channels.
E.g. the emission spectra of the fluorochromes CFP and YFP partially overlap,
and CFP signals also appear in the YFP fluorescence channel. This overlap can
be corrected by spectral unmixing.
Additional documentation in PDF format is available.
The public domain JAMA library,
a basic linear algebra package for Java,
is used for matrix inversion.
Authors: Olivier Marchal (marchal_olivier at hotmail.fr) and Jerome Mutterer (jerome.mutterer at ibmp-ulp.u-strasbg.fr) History: 2005/02/14: First version 2009/10/13: Emil Martinec fixed a bug in convolution between