LC_Pro is an imageJ plugin that
performs automated region of interest analysis (ROI) on gray
scale image sequences.
Start ImageJ and select
Plugins -> LCpro -> LCpro
Enter the ROI diameter in
Enter the movie frame rate (in
frames per second)
If you are measuring the
effect of a drug, check “drug treatment”
If “drug treatment”
is checked, enter the time interval during which the drug was
added (in seconds).
Select the detection
confidence limit: p = 0.05 or 0.01.
Select the folder containing
the files to be analyzed
a tab delimited file containing intensity versus time values for
each region of interest
a tab delimited file containing spatial and temporal information
about each detected event.
a tab delimited file containing the average parameter values
from LC_Pro. If “drug treatment” was checked, both
“Parameter List.txt” and “Mean Values.txt”
will be split into before/after versions of the files.
The file test.tif
is included as a demonstration. Running LC_Pro with default
parameters on test.tif should result in the output file
“Parameter List.txt” containing the following
1 230 32 1.50 13.75 9.12 0.88 8.25
2 254 38 1.47 14.75 6.38 1.12 5.25
Input files must be 8 bit,
grayscale .tif stacks with no scale information
Multiple files in a single
folder will be run sequentially using the entered parameter
Author: Joachim Walter (walter at biz.uni-muenchen.de) History: 2003/11/24: First version 2003/12/20: Adds “Scale:” option; macro recording works 2005/06/17: Improved and built into Image5D Source: Hypervolume_Opener.java
Author: Jeffrey Kuhn (firstname.lastname@example.org) The University of Texas at Austin Date: 2001/2/14 Source: Click_Forward_.java Installation: Copy Click_Forward_.class to the plugins folder and restart ImageJ. Description:
Author: Michael Abràmoff (http://bij.isi.uu.nl) Date: 2001/9/11 Source: Hypervolume_Shuffler.java Installation: Download Hypervolume_Shuffler.class to the plugins folder and restart ImageJ. Description: The plugin does various reconstruction operations
Author: Christopher Philip Mauer (cpmauer at northwestern.edu) History: 2004/07/15: First version Limitations: Only works with RGB images Source: RGB_Zprojector.java Installation: Download RGB_Zprojector.java to the plugins